Depends on the contents of the file you want to read and output in the list you want to get.
# assuming you have values each on separate line
values = []
for line in open('path-to-the-file'):
values.append(line)
# might want to implement stripping newlines and such in here
# by using line.strip() or .rstrip()
# or perhaps more than one value in a line, with some separator
values = []
for line in open('path-to-the-file'):
# e.g. ':' as a separator
separator = ':'
line = line.split(separator)
for value in line:
values.append(value)
# or all in one line with separators
values = open('path-to-the-file').read().split(separator)
# might want to use .strip() on this one too, before split method
It could be more accurate if we knew the input and output requirements.