Found the solution.
Private Sub dgRolloverInformation_ItemDataBound(ByVal sender As System.Object, ByVal e As System.Web.UI.WebControls.DataGridItemEventArgs) Handles dgRolloverInformation.ItemDataBound
'Hide "Delete" button for the first row in the RolloverInformation Datagrid
If (e.Item.ItemIndex = 0) Then
Dim btnDelete As LinkButton = CType(e.Item.Cells(4).FindControl("lnkbtnDelete"), LinkButton)
btnDelete.Visible = False
End If
End Sub