Yes you can by below way..
MediaMetadataRetriever retriever = new MediaMetadataRetriever();
try {
retriever.setDataSource(filePath);
//here 5 means frame at the 5th sec.
bitmap = retriever.getFrameAtTime(5);
} catch (Exception ex) {
// Assume this is a corrupt video file
}
For more info check it MediaMetadataRetriever