Open-source program to calculate the area of a bitmap [closed]

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I have just bought a Leica digital camera for a microscope. It was shipped with a software to take pictures and calibrate the pictures (pixel per mm) and it can measure length and imprint a ruler of 1mm in the file, but I want to measure areas too. The plugin to measure the area of a polygon should cost around 1000 EUR.

Hence I am looking for an open source program, which can measure the area of a polygon in a jpg (tif, png...) file.

For example the microscope picture shows the wing of a fly, I want to click a polygon around the wing with the mouse and get "4.32 mm^2"

If there is none, I will have to start it my own, but I am sure it is an FAQ and I use the wrong keywords in the search engine.

Answer 1

Engauge Digitizer supports to measure in bitmaps http://sourceforge.net/projects/digitizer/

Answer 2

What I tend to do is use the open-source vector drawing app 'inkscape' for this kind of jobs.

• import the image (File -> Import)
• draw the polygon you need to measure (Shift + F6)
• Choose from menu Extensions -> Visualize path -> Measure Path...
• Set the scale factor to the scale factor you know from calibration
• Set length units to mm
• Press 'Apply'. This will add an area indication as text field to the drawing

Answer 3

You can take photo and perform image analysis with Fiji (an ImageJ distribution). Other image analysis tool, Cellprofiler, can calculate objects features.

Micromanager project with Baumer optronics adapter can be handle with your camera too. Also check out this software list.