You can create a Constructor for that Class and you instantiate all Static variables there in your contructor just one time in your application:
Class Constants
Public Shared ReadOnly NLS_ALREADY_IN_USE_QUERYNAME As QNLSDefinition
'Constructor
Sub New()
NLS_ALREADY_IN_USE_QUERYNAME = New QNLSDefinition("AlreadyInUseQueryName", "Deutsch", "English")
End Sub
End Class
And then you go to your application constructor
Sub New()
InitializeComponent()
'Instantiate shared variables
Dim const as new Constants
End Sub()