You must sign each time. So change the assets folder, and build and sign and save, and then repeat the process.
There are alternatives you might consider:
- include all the assets and choosing the correct one at run time
- include the highest resolution image and scale the assets at run time.
Using either of these approaches you would require only one build, which makes everyone's life a lot easier.
Update, just thought of another alternative. Have a simple program with basic assets and download the correct assets for your device the first time the app is run. If these are large, the SD Card would be a good place for them.
Clarification
You use the same set of keys for all applications you build, they identify you as the developer, rather than being specific to one application. But you must sign every build that you plan to distribute.