Instead of using VisibleOnPageLoad try using the following code to open the window on itemclick.
protected void MainMenu_OnItemClick(object sender, RadMenuEventArgs e)
{
if (e.Item.Text == "About")
{
string script = "function f(){$find(\"" + RadWindow1.ClientID + "\").show(); Sys.Application.remove_load(f);}Sys.Application.add_load(f);";
ScriptManager.RegisterStartupScript(Page, Page.GetType(), "key", script, true);
}
}