I resolved this problem by using the secure port (636) in the connection string
<connectionStrings>
<add name="ADConnectionString" connectionString="LDAP://Server.domain.com:636/DC=domain,DC=com"/>
</connectionStrings>
And defining a valid username/password combo along with the SAM account and secure connection specifiers
<membership defaultProvider="ADMembershipProvider">
<providers>
<clear/>
<add name="ADMembershipProvider"
type="System.Web.Security.ActiveDirectoryMembershipProvider"
connectionStringName="ADConnectionString"
connectionUsername="XXX\Username"
connectionPassword="Password"
connectionProtection="Secure"
attributeMapUsername="sAMAccountName"/>
</providers>
</membership>
Thanks daren, your comments were helpfull.