You can keep track of connected users (and any associated metadata) by connection (check here and here for examples) and on your timer tick check your stored local data for whatever you need.
By itself, signalR won't pass you anything. The client has to pass things along.
If your client has the employee ID, have it send it to the signalr hub on connect. You can add a handler know when the client connects in your aspx page javascript, and then send it to the hub. The hub can then keep track of ConnectionId, EmployeeID
in a dictionary and you can use that to access back on the particular client or do whatever you want.
Check the links I posted, they show how to do this.