If I'm reading your question right, this should do what you want:
Dim sb As New StringBuilder(256)
sb.Append("128")
Or did you want spaces?
Dim sb As New StringBuilder("128".PadRight(256))
The 256 in the StringBuilder constructor is a performance optimization. Since you know the length of the resulting string, go ahead and reserve all the space for it up front. It's also possible you'll be doing a lot more work to this string, such that a different (larger) number or no number may be appropriate there.
Based on the comment:
Dim sb As New StringBuilder("128".PadRight(256, NullChar()))
But this is a really bad idea. Don't use nul characters in .Net.