Hmm, py.test 2.5.4 does not exist afaik.
Anyway, assuming you mean to ask whether it is possible to avoid the pytest-django plugin to test Django using py.test: the short answer is no.
The long answer is yes, but it is extremely difficult to get it all to work and you will basically write at least a minimal version of pytest-django in the conftest.py file. The pytest-django plugin was created specifically to work around all the weirdness which Django does with much global state and other hidden magic.
OTOH looking at the pytest-django source would probably help you kickstart such an effort. However you may consider thinking about what it is of pytest-django you do not like and maybe file an enhancement request to improve it.