You forgot your callback:
$("#amountHave").load("AmountRetrieve.aspx?desID=" + desID + "&qnty=" + qnty + " #amountHaveRet", function(){
if ($("#reverseDes").is(":checked") || $("#plain").is(":checked")) {
var slash = ".00/-";
var getAmnt = $("#amountHave").text();
getAmnt = getAmnt.replace(slash, '');
var finamAmount = parseInt(getAmnt, 10) + parseInt(getAmnt, 10);
$("#amountHave").text(finamAmount + slash);
}
else {
$("#blackholder").text("-------- Rs. " + $("#<%= qnty.ClientID %>").val());
$("#matchholder").text("-------- Rs. " + $("#<%= qnty.ClientID %>").val());
}
});