To load the data:
# open the file so you can read from it
with open("myfile.txt") as inf:
# for each line in the file,
# split it on commas (results in a list of strings)
# then convert each string to a float (results in a list of floats)
items = (map(float, line.split(",")) for line in inf)
# transpose (convert columns to rows),
# then assign each row to a variable)
xs, ys = zip(*items)
To plot it:
import matplotlib.pyplot as plt
plt.scatter(xs, ys)
plt.show()