Yes. It just works.
declare @t table (Dept varchar(20), Month varchar(20), [1st letter]varchar(20),[2nd letter]varchar(20),[3rd letter]varchar(20),[4th letter]varchar(20))
insert @t
SELECT 'ACCOUNTS' AS Dept
, DATENAME(MONTH, GETDATE()) AS [Month]
, '3254' AS [1st Letter]
, '2544' AS [2nd Letter]
, '1254' AS [3rd Letter]
, '64' AS [4th Letter]
SELECT * FROM @t AS t
unpivot (item for value in (Dept, Month, [1st letter],[2nd letter],[3rd letter],[4th letter])) u