But when i'm clicking the submit button it does nothing.
Since I don't see a Submit button, I'm taking a guess that you mean the Add and Remove buttons. If this is the case, the problem is that all your RequiredFieldValidators
have the ValidationGroup
property set to QuestionGroup but this is not set in any of your buttons.
Modify the Buttons like this:
<asp:ImageButton ID="btnAdd" runat="server" ValidationGroup="QuestionGroup" ... />