Autolayout was introduced at WWDC 2012 and is supported starting on iOS 6. It'll definitely not work with iOS 4.3.
I strongly recommend you to think about if you really need to support iOS 4.3 these days. We're probably very close to have iOS 7 now and you'll be years behind (technologically speaking) supporting iOS 4.3. I understand apps that are currently supporting iOS 4.3, but I don't think it's a good idea do start developing an app now with iOS 4.3 support in mind.