It depends on how and where you want to store it.
The REQUEST.file is a file object where you can read, seek, tell etc the contents from.
You can store it like a blob:
from ZODB.blob import Blob
blob = Blob()
bfile = blob.open('w')
bfile.write(REQUEST.file)
bfile.close()
# save the blob somewhere now
context.myfile = blob