In python, indentation matters. You're printing only last line. If you want to print each line, move your print inside the for look by indenting it:
in_file =open('tow.txt','r')
for line in_infile:
L=line.strip().split()
Tower = L[0]
Lat = L[1]
Long = L[2]
ComDis = L[3]
print (Tower,Lat,Long,ComDis)