That's over 1 day when I try to do it. Tried all ways which I know but sometimes you got stuck and can't do anything. I am so desperated. I even wrote a long thread to show everything what should be showed. If you have any suggestions, please comment it. That's last time. I promise.
That's my data:
> tbl_reo
id Sequence variable value
75 AAAAGAAAVANQGKK BiotinControl1_2 3893050.50
192 AAAAGAAAVANQGKK BiotinControl1_2 900604.61
3770 AAFTKLDQVWGSE BiotinControl1_2 90008.14
934 AAIKFIKFINPKINDGE BiotinControl1_2 656399.2021
1012 AAIKFIKFINPKINDGE BiotinControl1_2 359937.85
1970 AAIKFIKFINPKINDGE BiotinControl1_2 447089.3547
75 AAAAGAAAVANQGKK BiotinControl2 2717893.323
192 AAAAGAAAVANQGKK BiotinControl2 741299.3331
3770 AAFTKLDQVWGSE BiotinControl2 87127.07152
934 AAIKFIKFINPKINDGE BiotinControl2 646758.138
75 AAAAGAAAVANQGKK BiotinTreatment1_2 3435216.403
192 AAAAGAAAVANQGKK BiotinTreatment1_2 818936.8906
3770 AAFTKLDQVWGSE BiotinTreatment1_2 85120.95436
934 AAIKFIKFINPKINDGE BiotinTreatment1_2 1068384.547
The code which I am trying to use to calculate the p-value:
My Code:
tbl_anv <- tbl_all_onlyK[,c("id", "BiotinControl1_2", "BiotinControl2", "BiotinControl3", "BiotinTreatment1_2", "BiotinTreatment2", "BiotinTreatment3", "Sequence")]
tbl_reo <- melt(tbl_anv, measure.vars=2:7)
set.seed(1)
vars <- c("id", "BiotinControl1_2", "BiotinControl2", "BiotinControl3",
"BiotinTreatment1_2", "BiotinTreatment2", "BiotinTreatment3",
"Sequence")
tbl_reo <- as.data.frame(tbl_reo)
by(tbl_reo,tbl_reo$Sequence,function(x){
anova(lm(value ~ variable, data = x))$"Pr(>F)"[1]
})
An error ocurs:
There were 50 or more warnings (use warnings() to see the first 50)
Anyway, how can I do that and export the p-value in the separate column.
That's what I tried to do on my own:
aov_test <- by(tbl_reo,tbl_reo$Sequence,function(x){
anova(lm(value ~ variable, data = x))$"Pr(>F)"[1]
})
tbl_reo[,5] <- aov.test[[1]]$'Pr(>F)'[1]
Edit:
So I followed your suggestions and trying to do my best to show you my problems. So let's start with the head():
> dput(head(tbl_reo))
structure(list(id = c(75L, 192L, 3770L, 934L, 1012L, 1970L),
Sequence = structure(c(1L, 1L, 3L, 5L, 5L, 5L), .Label = c("AAAAGAAAVANQGKK",
"AAANILFSDHAD", "AAFTKLDQVWGSE", "AAIELRE", "AAIKFIKFINPKINDGE",
"AAIYKLLKSHFRNE", "AAKKFEE", "AAKYFRE", "AANVKKTLVE", "AARAGELLKE",
"AARDSKSPIILQTSNGGAAYFAGKGISNE", "AAVKARVASIDE", "AAVKASAPGSVILLE",
"AEKLKAE", "AEQVKKE", "AFAKRQGKE", "AFGSGTAAVVSPIKE", "AGIPAGVVNILPGSGRVVGE",
"AGISKDGQTRE", "AGSHGKDAWGVFRVHAFE", "AHSFSSSLASAE", "AIDALNGMLLNGQE",
"AITYSWTLLTE", "AKAKKAAKKAANAKQQQE", "AKALVAQGVKFIAE", "AKAQGVAVQLKRQPAQPRE",
"AKIGKKVE", "AKKVVKKAFEE", "AKNKKYALDIIKE", "ALANAGVTDIVLAVNYRPE",
"ALDFFARGLVKSPIKVVGLSTLPE", "ALFHPSVLGLE", "ALKIWKRFRE",
"ALKPDSQKSYAE", "ALLLKVNQIGTLSE", "ALRIGSE", "ALRLVE", "AMRIGSE",
"ANIIKLVE", "ANNKWNKLFSE", "ANVTGLRDAMFKGE", "AQKVRDQITSLKE",
"AQRVQAKNQLE", "ARINFGIEKAKE", "ASAGIQLSPKFVKLVSWYDNE", "ASFVLPTWAAKE",
"ASKLAAYNKKSTISARE", "ATSSIVMFSLNE", "ATTNAPWYKGWEKE", "ATVGAGLPIISFLRE",
"AVDATHLAE", "AVIRLSKE", "AVSKVYARSVYDSRGNPTVE", "AVVTVPAYFNDSQRQATKD",
"AVWYGPPKAANLGGVAVSGLE", "AWFPYQKE", "AYSPFGSANAPLLKE", "DGWVPGKNLFTVE",
"DHSFINYKQNVE", "DILYKIYKGVSE", "DLYQTFARTVE", "DNVKAHPRIE",
"DQVRNYVMQYIQE", "DSVYLAKLAE", "DVHNLFTYAKE", "DVKISAGTLLKGTE",
"DVQKFRHE", "EAVAKAAE", "EKPLFLVFHGGSGSTVQE", "EQRPGTPLFTVKAYLPVNE",
"ERIAALE", "FAKSLPRLDGLSVQE", "FALKHFPDYE", "FAPYGTITSAKVMRTE",
"FASLFPLKQAPAFLGPKGLKLTE", "FEQKNAGGVGE", "FFIDRPE", "FFIRDTKNLE",
"FGINTTVQE", "FGNRPMILHQIE", "FGVTKARWPAKSVKFVQGLLQNAAANAE",
"FIFSKYQILE", "FKMAKPE", "FKSMIKNNE", "FKTKYNSDPYQLE", "FLAADKE",
"FLKKILVDNKLE", "FLLSYGKE", "FMIAPTGAKTFAE", "FMISQLGLQKKNIKIHGF",
"FNRGLALE", "FNSQPDYRE", "FQKDAKRFE", "FQKTGPFE", "FRNGMINVSPIGRNASTEE",
"FRTASAQLE", "FSGSLLRSKFNTSNE", "FSRWFNHIASKADE", "FSTNVKDE",
"FTADGKVKPE", "FTPETPSPLIGILE", "FVADGLLQGFGNE", "FVAYPIQLLVTKE",
"FVAYPIQLVVTKE", "FVDIINSRN", "FVSSKHGSVSAE", "FYKSLKLPYRIVGIVSGE",
"GAAPGGFPGGAPPAPE", "GAIWNINSFDQWGVE", "GARYGPSLMPGGSEE",
"GEKTMGAKITKSLE", "GGLGPINIPLLADTNHSLSRD", "GGTLIGTARSME",
"GGVAPNIQTAEE", "GGVIIITHSAE", "GIFSGTLSYIFNE", "GIKVPIHIVQE",
"GILQRWTKGFDIPNIE", "GILQRWTKGFDIPNVE", "GILVASPFVELE", "GIPAGWQGLDNGPE",
"GIPPDQQRLIFAGKQLE", "GISKKTRIAGVVYHPSNNE", "GKKIATYQE",
"GKLKGVLGYTE", "GLDKIFQE", "GLGWGNNASAAIQRVGLGE", "GLNHLPVQKQIVE",
"GMMANGIAAGGQLTTTTE", "GMRWAGNANE", "GNDIHALAAKLLQE", "GQALKKKKGKSAE",
"GRGINSSYAVWNDVTGKAHE", "GRHSGYGKRKGTRE", "GSDVLNTWKKE",
"GSRKVDGQKVKASKE", "GTAVSQADVTVFKAFQSAYPE", "GTERSAYYTWAAE",
"GTRAVTKYSSSTQA", "GVVLGVEKRATSPLLE", "GWGTPHIKPYGNLSLDPSACVFHYAFE",
"GYSGVEVRVTPTKTE", "GYVGRTQPKKGGEGE", "GYWNGKITVDE", "HDHAGVPLKISPPVVAYRE",
"HIIQAKADAQRE", "HIVPGTYIQE", "HKFAIPAINVTSSSTAVAALE", "HKVVNGNSADAAYAQVE",
"HLQPHFTTNDGLE", "HNLRVISE", "HQIRAKFVE", "HQKYTREQVGCKE",
"IAKLNIPTGIPLVFE", "IAQHIFTKLNSNGYLEE", "IDASKPVKLTLLISE",
"IDPINALQAAME", "IDVAWLKANAKE", "IEAISSPLMGIFE", "IFDANVQRLKE",
"IFFKIKKTTPLRRLME", "IFGGYLYFAQAPSAAE", "IFGGYLYFAQAPSAAEFHTE",
"IFGPVVTVAKFKTLEE", "IFGPVVTVTKFKSADE", "IFLHSLPVKE", "IFSTYADNQPGVLIQVFE",
"IGATTSVFPFNKSMIEYLE", "IGNSGMFRPEMLE", "IGSLTRQGAKGGLLE",
"IHPLLPQDE", "IIAGNVATRE", "IIGGTTRIPTLKQSISE", "IIKKNFDLRPGVLVKE",
"IISNAKGQVVGSIIALDRQE", "IKGFFSFATQKLDE", "IKHLPFNVVNKDGKPAVE",
"IKKVVKAAAE", "IKLFNKWSFEE", "IKLVKLIQE", "ILIVTKDGVRKE",
"ILKIVPGRVSTE", "ILKKLDE", "ILLARNLI", "ILNKLALRILKNE", "ILNSQAPEE",
"ILSKLASAGATE", "ILWRQKE", "IMLPVFDAPQNLVE", "IMLQLGIPREE",
"IMLWNLAAKKAMYTLSAQDE", "INLPFITADASGPKHINMKFSRAQFE", "INLWFKKEE",
"IQLNGGSISE", "IQPRANIQLDFPE", "IRVFQGE", "ISAMVLTKMKE",
"ISDTMWPGQAMTLKVE", "ISDVFAKWNNGVLD", "ISLAAFGRKE", "ISTMIRWGLKPYLFVLNNDGYTIE",
"ITDIKQFLE", "ITHPTIVDGWFRE", "ITLGKYLFE", "ITQLMSLIINTVYSNKE",
"ITVDAPKAHE", "ITYLNNRAAAEYE", "IVERLNTASIPE", "IVFASPPRKSDGKVSPE",
"IVFNMSREE", "IVNLTLPDGTVRQGQVLE", "IVPKLME", "IVRVNKNAPAVE",
"IVSRFKTE", "IYEKME", "IYFSLNNGVRIPALGLGTANPHEKLAE", "IYNHIQLRE",
"IYNHIQLREE", "IYPYGSRGPKGLKE", "KAAKVTKAAQKAAKK", "KAALQTYLPKIKE",
"KAEQVFQNVKNILAE", "KAITYSHAAKE", "KAKIGQKE", "KAKKANNPQHSITKDE",
"KALTYTPPKKQKAEKPKAE", "KAQRAPLEE", "KAVAYWKTLKTDE", "KDFATFAKE",
"KDYASVVPPRHNE", "KEKLTQQKKE", "KFAAGTKALLDE", "KFINSEIE",
"KFNAVPIFLSDE", "KFSERLLNE", "KGAQTYVKKVIGSFKDWE", "KGCDIIVKNHE",
"KGLLSATQFTQPALTLME", "KGLSKKGGNIPMIPGWVMDFPTGKE", "KGQIVGRYVVDTSK",
"KGTGKSNKITITNDKGRLSKE", "KGVFRSIVPSGASTGVHE", "KGVFRSIVPSGASTGVHEALE",
"KGVITTQAE", "KGWVPVTKLGRLVKAGKITTIEE", "KIFTVPTETLQAVTKHFISE",
"KIGDLNFLIGPKLYE", "KKAADALLLKVNQIGTLSE", "KKAGKTLDVVE",
"KKAPAAAPAASKKKEE", "KKIDLASIRE", "KKPKTKKVKEE", "KKQAIRSKQE",
"KKTISLKTPLKTLVILHSDE", "KKVYPDVLYTSKLSRAIQTANIALE", "KKYIATIGVE",
"KLAAQRKAE", "KLIHGPKAQYNE", "KLIHNDSSYNHE", "KLKAAYSYMFDSLRE",
"KLKAERE", "KLKKVLSANTNAPFSVE", "KLSGIANNRDE", "KLVAVLK",
"KLYVGRAQKKNE", "KMIKPKEGRIE", "KMSSGYYLGE", "KMSTRAAPFEQRLPE",
"KNLFTGWVDVKLSAKGQQE", "KNLKPLAIIKGWGE", "KNMDAATALVGSGPAFVLLMLE",
"KNVPLYQHLAD", "KQLYSFDLE", "KQNLLAVE", "KRVSGMFLGE", "KSPFLDALKAKNFE",
"KTFVNVAKPFHKE", "KTKLQGMLNKAEE", "KTMGAKITKSLE", "KTQAFKKHVDE",
"KTVRKHLERQGWIE", "KVFNAYPAARE", "KVGLRLSPYGVFNSMSGGAE",
"KVILKMKE", "KVIRFQEE", "KVSVLKALGAE", "KYFPGLLRATNE", "KYSLAPVAKE",
"LAKKQKKLE", "LDALIAQGRE", "LDASNKAAKE", "LDLARPIYLPTASYGHFTNQE",
"LDTAQKHID", "LDTIRNME", "LEARIPE", "LFAKFGPIVSASLE", "LFAKHGE",
"LFDTHRVIVNE", "LFLKQLVVGGLDRVYE", "LFRSIGGE", "LGAVALKGALAKVPE",
"LGAVNLHE", "LGDKAVYAGE", "LGDNAVFAGE", "LGGEKKQKGQALQF",
"LGGKSPNIVFADAE", "LGGTVVSLSDSKGCIISE", "LGILPRDILTKE", "LGIYSHE",
"LGKGSFKYAWVLD", "LGKVLAKVIGKE", "LGMLAGADRVE", "LGTPFGVTIDFE",
"LHGNAKKAAEE", "LICSYRSKIETE", "LIGGSADLTPSNLTRWKE", "LIHQNEVPLVLLSSGVGVTPLLAMLE",
"LIHSKVGGE", "LIKLTGKLIE", "LILNGGFSPLTGFLNE", "LINNLGTIAKSGTKAFME",
"LKADLRPLQIKSIRE", "LKAHNINVVDK", "LKAQSKPHVGDE", "LKHLVGPEKAAE",
"LKIQRPLHE", "LKKKGILFVGSGVSGGEE", "LKKQKTAE", "LKKQQKEAE",
"LKKSANVGKKKE", "LKPTVILKLLKE", "LKRAIDLNKE", "LKRIMINCFNE",
"LKRIPE", "LKTNGKFQVDKSIIE", "LKYFGKALE", "LLAAFKAYLE", "LLAVPVVKGRKTEKE",
"LLAYQFASPVRWIE", "LLESPNNKVVPATNQIE", "LLKTNRISNE", "LLKTNRISNEQYE",
"LLLLDVAPLSLGIE", "LLLQQRATE", "LLRLVLLE", "LMAKLANDSPLAIE",
"LNAATGSKTSLYDKKE", "LNAAYAADGYARIKGMSCIITTFGVGE", "LNKGLTKKGGNIPMIPGWVMEFPTGKE",
"LNNAAAKKYDLE", "LNQLLKLVSE", "LPAWSKLQKIYE", "LPGVAFLSEKK",
"LPKNSTSSKDKKKKN", "LQAQLDYLNAE", "LQDIANPIMSKLYQAGGAPGGAAGGAPGGFPGGAPPAPE",
"LQGKLTGMAFRVPTVD", "LQGKLTGMAFRVPTVDVSVVD", "LRPLQFKSIRE",
"LRQATGGQAFPQMVFD", "LSALNGIAGSYAE", "LSFALIHKE", "LSFPFEWAFAGTNE",
"LSGIPPAPRGVPQIE", "LSKISGVDSSLIFPALE", "LSKSKTSPYVLPVPFLNVLNGGSHAGGALALQE",
"LSLLFNVIPDRYTKYD", "LSNVINMFFE", "LSQGTWLNKPKSVFQE", "LSRIGEFE",
"LTQFPAFVTPMGKGSIDE", "LTQLKKE", "LTSLTDYVTRMPE", "LTVKLNKE",
"LVARVQGGEE", "LVFGQTFTDHMLTIPWSAKE", "LVGRINGQFGTVE", "LVILGDSNVVRE",
"LVKVGHDNLVGE", "LVRDNKLIGNFTLAGIPPAPKGVPQIE", "LVRFSYGQDLE",
"LVSLPNIILTPHIGGSTEE", "LVTAPLDGTILE", "LYGNIVMSGGTTMFPGIAE",
"LYGTAIGARSQGAKTYLE", "LYPGRPLAIALD", "LYPGRPLAIALDTKGPE",
"LYSTSSSSTSSSATKN", "MATLYDGLE", "MAVAIKKE", "MAVKFGKNAF",
"MFFVSKVMNNE", "MFNDFLNAGAE", "MGQKKAPDGRIVE", "MIGSVVGIYNGKAFNQVE",
"MIGSVVGIYNGKAFNQVEIRPE", "MIISSPSDGQVKE", "MIVLHLPSPVTAQAYRAE",
"MKPTSIEKE", "MLAQDKLVAE", "MLGHYLGE", "MLGNWSFGDYFKKE",
"MLNRISPKILDE", "MLRAAGKE", "MNNIIAASRRH", "MPPQIPNE", "MQTAAQSSSIE",
"MQTEIKE", "NAHLKKNFKPQGSIE", "NALKLAKWLE", "NAVDRLLVE",
"NFAPFLKTILPE", "NFIQSIME", "NGFINNPIVISPTTTVGE", "NGHSPSEAFNE",
"NGITFPKDAPSPFIFKTLE", "NGIVTYAAKE", "NGLLSFVQYVIAPIQE",
"NGLTAYRLGKE", "NGSFLLYIDRHLVHE", "NGTVTAANASPINDGAAAVILVSE",
"NGVAKGSFKD", "NISNNYMIGAINAE", "NKERILTE", "NKIIRMPLVE",
"NKIVRKPLME", "NKKVIITGAPAAFSPTCTVSHIPGYINYLDE", "NKVQYIAGARPWTHVQKVDIALPCATQNE",
"NKVSPADAAKKAL", "NLHILE", "NLKDTSSGGVTHANE", "NLKKGSTVTSNGLNWE",
"NLKPSKPSYYLDPE", "NLLPLQSGIGNIANAVIE", "NLTVNPNVNE", "NMKLKLIEPLRE",
"NNIFNMVVE", "NNIFNMVVEIPRWTNAKLE", "NNMLHGNTMTVTGDTLAE",
"NNYKKRLLGTAFKSSPFGGSSHAKGIVLE", "NQGLKTATYPYWANE", "NQQGVNNFDE",
"NRGIKVANPVWSTIPFISE", "NRKISAVSTYFE", "NRNVGKTLVNKSTGLKNASDALKGRVVE",
"NRNVGKTLVNKSTGLKSASDALKGRVVE", "NRVKSDMLKE", "NSLLSSGTSQDSLRE",
"NTQLMNRDNIE", "NTSLSPKFVYQGE", "NVKTDSRNMPVKE", "NVQLYNGFVFHTGSLEE",
"NVQNGEVRFE", "NVRDKGNSALLE", "NVSSNIVKNAE", "NYNLLDTSGVAKVIE",
"NYTVERVNE", "PKLVLVRHGQSE", "PKLVLVRHGQSEWNE", "QAKLTAATNAKQ",
"QAVGGIYSVLNKKRGQVVSE", "QDKKKKSNH", "QDPSKSKVVVFE", "QDVPLIVPVVNPE",
"QGAQVLFASTDSE", "QGKPYISLPKGKGIKLSIAEE", "QGLKIDGPGFE",
"QHPRYGGVYVGTLSKPE", "QHPRYGGVYVGTLSKPEVKE", "QISSMVLGKMKE",
"QIVYALKLPGIIHIDAAE", "QLADYLIGVQY", "QLASQLVDLKKE", "QLGIHYE",
"QLYKNDSNNNNNNNGNNAE", "QQGKNATVSVEE", "QRPGTPLFTVKAYLPVNE",
"QRTKVEHVRE", "QSPRPGQQAFE", "QSPYVSWVSYPGLASHSHHE", "QSTKFGTEIITE",
"QTYKKPTGGIVTVRSE", "QVADISSAKVNFKSLE", "QVAKWVNLAQKE", "RAVPHGKWQDE",
"RDLIKKKTKNNE", "RFLNVFPKLVEE", "RGEIKKGE", "RGLPVKLTYTDNKTSKE",
"RHIQVGDGLE", "RLAIVGVE", "RLLWYLARE", "RLNTASIPE", "RLNTASIPENVE",
"RLTSLNVVAGSDLRRTSIIGTIGPKTNNPE", "RLVAIAEQD", "RMHVLKKQYE",
"RNAAYQKE", "RNAAYQKEYE", "RNKIHQYLFQE", "RQLNAVLE", "RQLNAVLEE",
"RTSLFLNLANDPTIE", "RTVLFPIKYHE", "RVKANAAKRAE", "RYKLVPGIFADVKNLKE",
"RYKYVDPNVLPE", "RYVFLLDPMLATGGSAIMATE", "SALSYAALILADSE",
"SCKMPQTVE", "SDKSKWLTGVE", "SEYWPRLTKE", "SFAANWGVMVSHRSGETE",
"SFQAVYNKLTGKQIVFE", "SGSKLKFYVLHGDGVE", "SGTLGTKGNTQVIIPRLTE",
"SGTVFDSSYSRGSPIAFE", "SHIGVAKKLE", "SHLIPSATTGE", "SIAVLSAMKME",
"SIAYSLKNTISE", "SIQKTKE", "SIRVGEYNAE", "SISDSLSSKPHNFE",
"SKFAKAYAQGVSKKE", "SKFKGDITWLPVRRKAYWE", "SKLPTYTAKDSAVATRKLSE",
"SLDQAWSLLRIYPKE", "SLGSPSGATKARIVVAKSGEL", "SLIDAKTGLPKE",
"SLKTGNAGPRPACGVIGLTN", "SLQNKGQEVKE", "SLRGLGGILLNPITGRRFVNE",
"SLSSKLSVQD", "SNLKKITLE", "SNPTAKLNLIWPATPIHIKKYE", "SQGKTLSVKQE",
"SQLTNLFNEE", "SQRIASKNQLE", "SRFQEIVKE", "SRKLFAATVAKAKTIVWNGPPGVFE",
"SSANRKIVKE", "STKNGTETKRSLE", "STPDTPSPLIAVNE", "STVAGFLVGSE",
"STVLQFKE", "SVKLTKAVAE", "SVLAHL", "SYQKSQKSQKE", "SYVDKHPVVTFNQE",
"TAGLTVGDPVLRTGKPLSVE", "TAIGPSGQLKGYLRPE", "TAKGNYPINAVTTMAE",
"TAQGQFLNFNKLLE", "TARSTATGPSEAVWYGPPKAANLGGVAVSGLE", "TDPFKLSGAQVVD",
"TEPLGTAGPLKLAE", "TFKLFNKYRPE", "TFRTTNTL", "TGGPISVPVGRE",
"TGIVAQYAYVLGELE", "TGVIKPGMVVTFAPAGVTTE", "THINLKVSDGSSE",
"TIGNPKYNVPDFE", "TIQDKSSAGAVVVANAKSLE", "TKAGVVKGKTLLE",
"TKKSCCSGK", "TKNFTPEQISSMVLGKMKE", "TKTFSPQE", "TKYLSGIAKRLNKE",
"TLKKFGEE", "TLKQLNASLADKSYIE", "TLKVVDPE", "TLRKVVKHFIDE",
"TMKAVVIE", "TMWDTKKE", "TNPGTDVTVSSVE", "TQALLLAPIAPHFAE",
"TQKGVIFYE", "TRLSLTHMVE", "TSGSTVNDPLANYE", "TSNFIKKVGYNPKTVPFVPISGWNGDNMIE",
"TSSKKPKHPLE", "TTAMITDIATAPAE", "TTHIKFASDPGCAFTKSIGFE",
"TTTGVHHLYRMVKE", "TVAASAVAAVFE", "TVAKSKNAE", "TVFAGQKP",
"TVLRQALGE", "TVQVNLPVSLE", "TVSKMAYLDKTGE", "VAAIMQDPVMQSILQQAQQNPAALQE",
"VAALAAENK", "VAKVVGANPAAIKQAIAANA", "VANPIMSKLYQAGGAPE",
"VARVILTQVGSGPQETNE", "VATRAKQGELLE", "VDASGFRIPQQE", "VDSVLKHMKE",
"VEGMRWAGNANE", "VFIDFTKE", "VFKFFGFTPE", "VFKFFGFTPEGVAE",
"VFLNEHPE", "VFVATKE", "VGAKGVQLLSSYITEE", "VGASMTRSIQTLE",
"VGKSVAVDSSE", "VGLKRVVTKAMSSR", "VGTMNVFFVFLNKVTGKKE", "VGVHVLPKILDE",
"VHFVSNIDGTHIAE", "VIAGNVVTRE", "VIDTILALVKD", "VKILNGFAFVE",
"VKVVLFNHSQRD", "VLANYLTSALSE", "VLAVQKKLIAKSNLAGKPVICATQMLE",
"VLGPFAFRIGSVKE", "VLIKRGVKPE", "VLLLDVTPLSLGIE", "VLLVAVGRRPYIAGLGAE",
"VLNPQIIKDVLE", "VLPRAVGSLTFDE", "VLVLRGTLE", "VLVVRGSKKGQE",
"VLYTSKLSRAIQTANIALE", "VMSIGRTFEE", "VMTKNPVTGIKGITLKE",
"VQNSTLAQLTSKLIPE", "VQSAVLGFPRIGPNRE", "VRVTPTKTE", "VSAAYKNSLE",
"VSDVAQKAE", "VSGLQYIDLLLIHSPLE", "VSNPNIIFFRGTYAAVSPE",
"VSNPPAYGAKIVAKLLETPE", "VSNRSTPSVVGFGPKNRYLGE", "VSSMPTLIFYKGGKE",
"VSVVDLTVKLNKE", "VTAALRVTD", "VTPLVQNVTGDKE", "VTRVVGANPAAIKQAIASNV",
"VTSSFFYRGNYTDFE", "VVALNDPFITND", "VVKILRE", "VVLPVDFIIAD",
"VVLTHPADE", "VVNIGIGGSDLGPVMVTE", "VVTSASAPPENASE", "VVVITKNVSGQDVAAALE",
"VYHNLKSLTKKRYGASAGNVGDE", "VYKIGGIGTVPVGRVE", "WAGLGKIDRE",
"WFDIKAPSTFE", "WFRIYKIPDGKPE", "WGKFLTCVEVE", "WLFKLREE",
"WMWNQLGNFLQE", "WSKLISE", "WSLTAPLGKALHDLS", "WTKIFKAIHE",
"YAQLLAKRLSE", "YAQLLAKRLSERKAE", "YFATHITE", "YFATHITERE",
"YFQLLNSALTE", "YGYSARVVDLIE", "YGYSTRVVD", "YGYSTRVVDLVE",
"YIALVDQLIAKYSS", "YIASKKGSISAE", "YKDIPVPKPKANE", "YKPLFTE",
"YLLKAIKE", "YLNLPEHIVPGTYIQE", "YNKIGDILSGRLKLRAE", "YQSKIIVFPRDGKAPE",
"YQTQVLKNAKALE", "YQYLRSVVNE", "YRKVLPIVSVPE", "YRLSVLPDNVPIMSVE",
"YSLLAWTNIPRKE", "YVAKVSARE", "YVNKGQDFHIAGE", "YVPSVIEPSFGIGRIIYSVFE",
"YVQAAKNSIAAGADGVE"), class = "factor"), variable = structure(c(1L,
1L, 1L, 1L, 1L, 1L), .Label = c("BiotinControl1_2", "BiotinControl2",
"BiotinControl3", "BiotinTreatment1_2", "BiotinTreatment2",
"BiotinTreatment3"), class = "factor"), value = c(3893050.501,
900604.6087, 90008.14298, 656399.2021, 359937.85, 447089.3547
)), .Names = c("id", "Sequence", "variable", "value"), row.names = c(NA,
6L), class = "data.frame")
Now something about warnings. I believe that is something meaningfull wrong with my code.
1: In anova.lm(lm(value ~ variable, data = x)) :
ANOVA F-tests on an essentially perfect fit are unreliable
2: In anova.lm(lm(value ~ variable, data = x)) :
ANOVA F-tests on an essentially perfect fit are unreliable
3: In anova.lm(lm(value ~ variable, data = x)) :
ANOVA F-tests on an essentially perfect fit are unreliable
4: In anova.lm(lm(value ~ variable, data = x)) :
ANOVA F-tests on an essentially perfect fit are unreliable
And that's a simple function which I want to use in the loop:
aov.test <- summary(aov(tbl_reo$value_column ~ as.factor(tbl_reo$variable)))
tbl_reo[,5] <- aov.test[[1]]$'Pr(>F)'[1]
Is there anyone who can help me with that ? Just asking for the last time and sorry for this little spam.
https://stackoverflow.com/questions/19586113
italiano
english
français
española
中国
日本の
العربية
Deutsch
한국어
Português
Russian