If you have ran it by mistake and you want to stop it without writing an extra script and without restarting your server. So here is a trick.
First, uninstall gunicorn
sudo pip3 uninstall gunicorn # I am using python3 in ubuntu server so I am using sudo and pip3
Then kill the process running for your app's port number (I am assuming port number as 8000 by default).
To get the process number in which your app is running use this:
sudo lsof -i:8000
Result
COMMAND PID USER FD TYPE DEVICE SIZE/OFF NODE NAME
gunicorn 17XX95 root 6u IPv4 155XXXX 0t0 TCP localhost:8000
gunicorn 17XX97 root 6u IPv4 155XXXX 0t0 TCP localhost:8000
Then just kill the processes, it is possible that you can get more than one process ids(PID), see the result above.
sudo kill 17XX95 17XX97
Now if you want to reinstall gunicorn you can install it
sudo pip3 install gunicorn