this is possible and can be easy in python.
Pyaudio is a nice library and you can use to play your audio!
First do you need decode the audio file (wav, mp3, etc) this step convert audio data in numbers(short int or float32).
Do you need convert the seconds in equivalent position point to cut the signal in the position of interest, to do this multiply your frame rate by what seconds do you want !
Here one simple example for wav files:
import pyaudio
import sys
import numpy as np
import wave
import struct
File='ederwander.wav'
start = 12
length=7
chunk = 1024
spf = wave.open(File, 'rb')
signal = spf.readframes(-1)
signal = np.fromstring(signal, 'Int16')
p = pyaudio.PyAudio()
stream = p.open(format =
p.get_format_from_width(spf.getsampwidth()),
channels = spf.getnchannels(),
rate = spf.getframerate(),
output = True)
pos=spf.getframerate()*length
signal =signal[start*spf.getframerate():(start*spf.getframerate()) + pos]
sig=signal[1:chunk]
inc = 0;
data=0;
#play
while data != '':
data = struct.pack("%dh"%(len(sig)), *list(sig))
stream.write(data)
inc=inc+chunk
sig=signal[inc:inc+chunk]
stream.close()
p.terminate()