One line solution (for each image) is to set the axis as the parent of the image within the imshow command;
imshow(image_Data,'Parent',handles.axes1)
There should be no need to open the additional figure windows ( assuming the axes are with the gui...)
So specifically for the question above:
imshow(s1,'Parent',handles.axes3);
imshow(s2,'Parent',handles.axes4);
imshow(s2&s1,'Parent',handles.axes5);