I think you mean you have two sample populations, right? if you have two samples that means you have only 2 'records'. Standard error refers to a "standard devation" that occurs with in a sample population. A standard deviation refers to a complete population. Significance is usually derived with a formula that helps us understand if an 'effect' is from chance alone.
Also, if you really do meant that you only have two samples, it is not enough for statistical research. Most large studies use thousands of samples. However, for "statistical" purposes they taught me in a math class that 36 is generally acceptable, as long as theres no bias and it's for school-work. So.. I hope this helps.
edit: oh, a standard deviation indicated the "spread" of the data.. http://upload.wikimedia.org/wikipedia/commons/thumb/8/8c/Standard_deviation_diagram.svg/350px-Standard_deviation_diagram.svg.png