Old School: http://jsfiddle.net/PAGUp/
var elem = document.getElementById('targetImg');
var oldSrc;
elem.onmouseover = function() {
oldSrc = elem.src;
elem.src = 'http://www.eclipse-developers.com/images/up_hover.png';
}
elem.onmouseout = function() {
if(typeof oldSrc !== 'undefined') {
elem.src = oldSrc;
}
}
I'm sure the jquery is more pithy. Essentially you need a variable to hold the 'old' src URL, and mouseover and mouseout handlers to set the new URL and back it out.