The code danyo poste would work fine, but on the route you chose with defining templateDir as a javascript variable you would have :
$(this).html('<img src="' + templateDir + '/images/pauze-play.png" />');
Notice: there are no spaces between "' and '/ . This might have been where you went wrong.
Also you might want to declare templateDir as a global variable instead. so you would have:
var templateDir = '<?php bloginfo("template_directory"); ?>';
jQuery('.selector').click(function(){ ...