The explanation of your problem is very well given by floris.But here due to lack of full your code we are not able to perform it on our system. you can aslo try for
axis ij
which is some what similar to above answer. you can also try the below code.
imshow(inFrame,'Parent',handles.axes8);
hold on;
p1 = plot([c],[r],'r.');
set(p1,'Parent',handles.axes8);
hope this works for you..let me know about the result..