I am not sure of you have the error in the CreateDirectory call or afterward. I am sure that the FileUpload control doesn't have a SaveAs method and this is on the PostedFile object and that you need to pass a FileName not a Directory name
Dim directoryPath As String = Server.MapPath("~/bussnisses")
If Not Directory.Exists(directoryPath) Then
Directory.CreateDirectory(directoryPath)
End If
Dim destFile = Path.GetFileName(FileUpload1.PostedFile.FileName)
destFile = Path.Combine(directoryPath, destFile)
FileUpload1.PostedFile.SaveAs(destFile)
Label1.Text = "complete"