Configuration error in Apache

Question

When i created server In Apache 2.2 on Windows Server 2008, Python 3.3 and Django 1.5. It give me this error why? This is in my conf:

WSGIScriptAlias / D:/Rocnikova_prace/mysite/mysite/wsgi.py
WSGIPythonPath D:/Rocnikova_prace/mysite

<Directory D:/Rocnikova_prace/mysite/mysite>
<Files wsgi.py>
Order deny,allow
Require all granted
</Files>
</Directory>

[crit] [client 127.0.0.1] configuration error: couldn't perform authentication. AuthType not set!: /hlavni_stranka/

Answer 1

Ok i Know where was the problem, It was in configuration:

<Directory D:/Rocnikova_prace/mysite/mysite>
<Files wsgi.py>
Options FollowSymLinks
AuthType None
AllowOverride None
Order deny,allow
Deny from all
</Files>
</Directory>

But it give me new error:

 [Sun Nov 10 13:07:13 2013] [error] [client 127.0.0.1] client denied by server configuration: D:/Rocnikova_prace/mysite/mysite/wsgi.py

Why?