Ok i Know where was the problem, It was in configuration:
<Directory D:/Rocnikova_prace/mysite/mysite>
<Files wsgi.py>
Options FollowSymLinks
AuthType None
AllowOverride None
Order deny,allow
Deny from all
</Files>
</Directory>
But it give me new error:
[Sun Nov 10 13:07:13 2013] [error] [client 127.0.0.1] client denied by server configuration: D:/Rocnikova_prace/mysite/mysite/wsgi.py
Why?